An Integrative Apoptotic Reaction Model for extrinsic and intrinsic stimuli

This is a first author publication from my PhD project.

For this paper, I studied the apoptotic cascade through the use of pseudo homo-FRET sensors that probe the integral activity of intrinsic, extrinsic and effector caspases simultaneously in single cells. To avoid experimenter bias and amass enough data, I worked on the design of experiments and programming of Python modules that automatically analyze acquired images. After my previous work, I looked into applying intrinsic and extrinsic apoptotic stimuli to have a holistic view of the whole cascade. I developed apoptoside to analize the polarization anisotropy curves, estimate enzymatic activity and timing between maximum activity of caspases. An ordinary differential equation model capable of describing both scenarios, as well as previous publications from other groups, was developed an uploaded to Biomodels and my GitHub.